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KMID : 0364820080440020147
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Korean Journal of Microbiology
2008 Volume.44 No. 2 p.147 ~ p.155
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Comparative Analysis of Dissimilatory Sulfite Reductase (dsr) Gene from Sediment of Lake Sihwa, Korea and Lake Aha, China
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Kim In-Sun
Ahn Tae-Seok
Kim Ok-Sun
Jeon Sun-Ok
Karl-Paul Witzel
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Abstract
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The diversity of sulfate reducing bacteria was investigated in different depths of sediments in Lake Sihwa,
Korea and Lake Aha, China by PCR amplification, denaturing gradient gel electrophoresis (DGGE) and clone
libraries targeting dissimilatory sulfite redectase (dsr) gene. In the analysis of DGGE band patterns, the community
compositions of dsr gene in the sediments of both lakes were significantly different whereas bands in all
depths of each environment revealed similar patterns. Bands from Lake Sihwa were produced much more than
those from Lake Aha, demonstrating a higher diversity of dsr gene in Lake Sihwa. Total 68 clones containing
dsr gene were obtained to analyze their sequences. Sequences from the sediment of Lake Sihwa were affiliated
to Deltaproteobacteria, the Gram-positive thermophilic sulfate reducers belonging to the genus Desulforomaculum
and archaeal thermophilic SRB belonging to the genus Archaeoglobus, whereas sequences from the
sediments of Lake Aha were related to genus Desulfotomaculum. Clones retrieved from sediment of Lake
Sihwa revealed a higher numbers than those of Lake Aha, demonstrating a higher diversity of dsr gene in Lake
Sihwa. Most of clones (59%) were distantly related to the known cultivated SRB with 60~65% of similarity,
which were clustered only the sequences from the environments showed less than 90% similarity. These habitat
specific sequences suggested that the clustered dsr sequences represent species or groups of species that were
indigenous to these environments. This study showed that these lakes have a specific bacterial communities having
dsr gene distinct from those in other environments such as soil and marine ecosystems around the world.
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KEYWORD
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dsr gene, lake Aha, lake Sihwa, sediment, sulfate reducing bacteria (SRB)
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